Optimization of Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Milk and Colostrum of Naturally Infected Dairy Cows
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منابع مشابه
Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods
Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...
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Abstract Background and objectives: Paratuberculosis or John's disease is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). It results in major economic losses to dairy farm of all over the world and it is the agent causing crohn's disease. The aim of this study was to detect the MAP using PCR in raw-milk samples of cows in shahre-kord. Mater...
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The efficacy of bacterial cultures and IS900-specific polymerase chain reaction (PCR) was compared for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) from the mesenteric lymph nodes of goats. Samples were collected from 75 goats slaughtered in Ilam, in southwest of Iran. Tissue homogenates were inoculated onto four media. The genomic DNA was extracted directly from mesenteri...
متن کاملColostrum and milk as risk factors for infection with Mycobacterium avium subspecies paratuberculosis in dairy cattle.
Mycobacterium avium ssp. paratuberculosis (MAP) infections cause major losses to the dairy industry. Transmission of MAP occurs primarily via feces and in utero, but MAP can also be excreted in colostrum and milk. The objective of this study was to determine whether colostrum and milk fed to calves are important risk factors for infection with MAP. A questionnaire was sent to 1,050 farms partic...
متن کاملThermal Inactivation of Mycobacterium avium subsp. paratuberculosis in Artificially Contaminated Milk by Direct Steam Injection.
UNLABELLED The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected with M. avium subsp. paratuberculosis. We also tested milk artificially contamin...
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